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KMID : 0903619980390060838
Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 6 p.838 ~ p.842
Growth and Leaf-surface Characteristics of Chrysanthemum Plantlets between Micropropagation and Microponic System
ÀÌ¿ë¹ü/Lee, Yong Beom
¹èÁ¾Çâ/ÇÑÀºÁÖ/Bae, Jong Hyang/Hahn, Eun Joo
Abstract
Chrysanthemum (Dendranthema grandiflorum ¢¥Bongwhang¢¥) shoots induced in vitro were cut by 1-1.5 §¯ nodes and grown by two different culture systems conventional microprapagation and microponic system which combines micropropagation with hydroponics. Growth and survival of the plantlets were observed 30 days after planting. Chrysanthemum plantlets grown in the microponic system showed much higher rate in leaf length (5.83 §¯), leaf width (4:14 §¯), and leaf area (45.02 §²) than those grown in vitro (1.78 §¯, 1.13 §¯ and 6.3 §¯2, respectively). Higher fresh and dry weight were also observed in the microponic system. More leaves were observed in vitro (12.2 per plantlet) than in microponics (10.1) but the leaves were very small and did not fully expand. One hundred per cent of plantlets in both systems survived after transplanting. Photosynthetic rates of the plantlets were higher in the microponic system than in vitro. In anatomical comparisons, in vitro-grown leaves showed greater cell and stomatal densities than the leaves in the microponic system. However, greater cell size and regular cell shape were observed in the microponic system. These results indicated that chrysanthemum plantlets in the microponic system could grow better with shorter period than those in the micropropagation system.
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